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mouse c3a elisa kit  (Novus Biologicals)


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    Novus Biologicals mouse c3a elisa kit
    Mouse C3a Elisa Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+c3a+elisa+kit/Mouse+Complement+C3a+ELISA+Kit+(Colorimetric)/pm41963079-229-15-19
    Average 94 stars, based on 12 article reviews
    mouse c3a elisa kit - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: CD55-expressing myeloid-derived suppressor cells (MDSCs) drive cancer immunoevasion
    Article Snippet: Total RNA was extracted from cells using the EZ-press RNA Purification Kit (EZBioscience) according to the manufacturer’s protocols, followed by reverse transcription into cDNA using 4×Reverse Transcription Master Mix (EZBioscience). qPCR was then performed with the synthesized cDNA using 2×SYBR Green qPCR Master Mix (EZBioscience). .. The concentrations of complement components C3a and C5a in tumor tissues were measured using the Mouse C3a ELISA Kit (Novus Biologicals) and Mouse C5a ELISA Kit (MyBioSource), following the manufacturer’s instructions. .. For myeloid and lymphoid cell analysis, all samples were incubated with anti-CD16/32 (BD Pharmingen) at 4°C for 30 min to block Fc receptors and then stained with antibodies against cell surface antigens.

    Article Title: Benzo nitrogen-containing heteroaromatic ring derivative and use thereof in medicine
    Article Snippet: .. The plasma C3a level was detected using mouse C3a ELISA kit (NOVUS/NBP2-70037), and the inhibition rate of C3a was calculated. ..

    Article Title: CD55-expressing myeloid-derived suppressor cells (MDSCs) drive cancer immunoevasion.
    Article Snippet: Total RNA was extracted from cells using the EZ- press RNA Purification Kit (EZBioscience) according to the manufacturer’s protocols, followed by reverse transcription into cDNA using 4×Reverse Transcription Master Mix (EZBioscience). qPCR was then performed with the synthesized cDNA using 2×SYBR Green qPCR Master Mix (EZBioscience). .. The concentrations of complement components C3a and C5a in tumor tissues were measured using the Mouse C3a ELISA Kit (Novus Biologicals) and Mouse C5a ELISA Kit (MyBioSource), following the manufacturer’s instructions. .. For myeloid and lymphoid cell analysis, all samples were incubated with anti- CD16/32 (BD Pharmingen) at 4°C for 30 min to block Fc receptors and then stained with antibodies against cell surface antigens.

    Clinical Proteomics:

    Article Title: Benzo nitrogen-containing heteroaromatic ring derivative and use thereof in medicine
    Article Snippet: .. The plasma C3a level was detected using mouse C3a ELISA kit (NOVUS/NBP2-70037), and the inhibition rate of C3a was calculated. ..

    Inhibition:

    Article Title: Benzo nitrogen-containing heteroaromatic ring derivative and use thereof in medicine
    Article Snippet: .. The plasma C3a level was detected using mouse C3a ELISA kit (NOVUS/NBP2-70037), and the inhibition rate of C3a was calculated. ..



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    Inhibition of C3aR ameliorates rotenone-induced neurodegeneration, synaptic loss, α-synuclein phosphorylation and cognitive deficits in mice. C57BL/6 mice were treated with rotenone for 3 weeks to establish PD models, with 30 min of SB290157 (C3aR inhibitor, 1 mg/kg, i.p.) administration after each rotenone injection. (A) Concentrations of C3a in the hippocampus of Con and Rot mice, measured by enzyme-linked immunosorbent assay (ELISA). n = 6. (B) Representative Western blot images and densitometric quantification of C3aR protein levels in the hippocampus and rotenone mice. n = 4. (C) The mRNA levels of C3aR in vehicle and rotenone mice. (D) Representative images of double-immunofluorescence staining with C3aR and Iba-1 or Neu-N antibodies and (E, F) the quantification of C3aR + Iba-1 + and C3aR + Neu-N + cells in vehicle and rotenone mice. n = 3. (G) The quantification of Neu-N + cell number and (H) optical density of PSD95 staining in rotenone mice with or without C3aR inhibitor treatment. (I) The representative images of Neu-N and PSD95 staining in rotenone mice with or without C3aR inhibitor treatment. n = 6. (J, K) Representative bands of Neu-N, PSD95 and ser129-phosphorylated α-synuclein and the quantification of blots in rotenone mice with or without C3aR inhibitor treatment. n = 4. (L) Representative images of ser129-phosphorylated α-synuclein and (M) the quantification of staining density in rotenone mice with or without C3aR inhibitor treatment. n = 3. (N–Q) Escape latency, traveled distance, first platform crossing latency and platform crossing number in rotenone mice with or without C3aR inhibitor treatment. n = 15; ∗ p < 0.05, ∗∗ p < 0.01 for comparison between Rot & Rot + C3aR inhibitor groups; Scale bar = 50 μm.

    Journal: Redox Biology

    Article Title: The C3–C3aR axis drives rotenone-induced cognitive damage via synaptic engulfment, dark microglia and PANoptosis

    doi: 10.1016/j.redox.2026.104062

    Figure Lengend Snippet: Inhibition of C3aR ameliorates rotenone-induced neurodegeneration, synaptic loss, α-synuclein phosphorylation and cognitive deficits in mice. C57BL/6 mice were treated with rotenone for 3 weeks to establish PD models, with 30 min of SB290157 (C3aR inhibitor, 1 mg/kg, i.p.) administration after each rotenone injection. (A) Concentrations of C3a in the hippocampus of Con and Rot mice, measured by enzyme-linked immunosorbent assay (ELISA). n = 6. (B) Representative Western blot images and densitometric quantification of C3aR protein levels in the hippocampus and rotenone mice. n = 4. (C) The mRNA levels of C3aR in vehicle and rotenone mice. (D) Representative images of double-immunofluorescence staining with C3aR and Iba-1 or Neu-N antibodies and (E, F) the quantification of C3aR + Iba-1 + and C3aR + Neu-N + cells in vehicle and rotenone mice. n = 3. (G) The quantification of Neu-N + cell number and (H) optical density of PSD95 staining in rotenone mice with or without C3aR inhibitor treatment. (I) The representative images of Neu-N and PSD95 staining in rotenone mice with or without C3aR inhibitor treatment. n = 6. (J, K) Representative bands of Neu-N, PSD95 and ser129-phosphorylated α-synuclein and the quantification of blots in rotenone mice with or without C3aR inhibitor treatment. n = 4. (L) Representative images of ser129-phosphorylated α-synuclein and (M) the quantification of staining density in rotenone mice with or without C3aR inhibitor treatment. n = 3. (N–Q) Escape latency, traveled distance, first platform crossing latency and platform crossing number in rotenone mice with or without C3aR inhibitor treatment. n = 15; ∗ p < 0.05, ∗∗ p < 0.01 for comparison between Rot & Rot + C3aR inhibitor groups; Scale bar = 50 μm.

    Article Snippet: The C3a ELISA kit was purchased from CUSABIO (CSB- E08511 m, Houston, TX, USA), and TUNEL assay kit was provided by KeyGEN (KGA7073-1, Nanjing, China.

    Techniques: Inhibition, Phospho-proteomics, Injection, Enzyme-linked Immunosorbent Assay, Western Blot, Double Immunofluorescence Staining, Staining, Comparison